CE: 1631 CF: cag011192205
RESEARCH ARTICLE
Conidial production and conservation of the isolate Ma-005 of Metarhizium anisopliae (Metsch.) Sorokin
Producción y conservación de conidios del aislado Ma-005 de Metarhizium anisopliae (Metsch.) Sorokin
Irma García Cruz*, Elio Minel del Pozo Núñez*, Yoisel Hernández Pérez
Universidad Agraria de La Habana (UNAH) "Fructuoso Rodríguez Pérez", Carretera a Tapaste y Autopista Nacional km 23 ½, San José de Las Lajas, Mayabeque, Cuba, CP 32700
*Autor para correspondencia: This email address is being protected from spambots. You need JavaScript enabled to view it.; This email address is being protected from spambots. You need JavaScript enabled to view it.
ABSTRACT
This work was carried out in the Plant Protection Laboratory at the Agronomy Faculty of the Agrarian University of La Habana and its objective was to evaluate mass production and conservation of isolate Ma-005 belonging to the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorokin, which was obtained from a soil sample of the "El Guayabal" property, in the municipality of San José de las Lajas, using the insect bait technique. In this study, it was evaluated: biomass production in liquid culture media, production of conidia in solid substrates and conidia conservation in different oils. The greatest biomass production was reached when the molasses-yeast ratio in the substrate was 10:30 or 20:30 g L-1 with an incubation time of 72 h. Among the evaluated solid substrates, rice and sorgo were the most favorable for conidia production. All used oils kept the conidia viability over the 70 % for six months.
